Facile construction of mutations inHaemophilus ducreyiusinglacZas a counter-selectable marker
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چکیده
منابع مشابه
Recombineering with tolC as a Selectable/Counter-selectable Marker: remodeling the rRNA Operons of Escherichia coli
This work describes the novel use of tolC as a selectable/counter-selectable marker for the facile modification of DNA in Escherichia coli. Expression of TolC (an outer membrane protein) confers relative resistance to toxic small molecules, while its absence renders the cell tolerant to colicin E1. These features, coupled with the lambdaredgam recombination system, allow for selection of tolC i...
متن کاملCounter-selectable marker for bacterial-based interaction trap systems.
Counter-selectable markers can be used in two-hybrid systems to search libraries for a protein or compound that interferes with a macromolecular interaction or to identify macromolecules from a population that cannot mediate a particular interaction. In this report, we describe the adaptation of the yeast URA3/5-FOA counter-selection system for use in bacterial interaction trap experiments. Two...
متن کاملmazF, a novel counter-selectable marker for unmarked chromosomal manipulation in Bacillus subtilis
Here, we present a novel method for the directed genetic manipulation of the Bacillus subtilis chromosome free of any selection marker. Our new approach employed the Escherichia coli toxin gene mazF as a counter-selectable marker. The mazF gene was placed under the control of an isopropyl-beta-D-thiogalactopyranoside (IPTG)-inducible expression system and associated with a spectomycin-resistanc...
متن کاملThe chicken HPRT gene: a counter selectable marker for the DT40 cell line.
We describe the cloning, characterisation and chromosomal mapping of the chicken hprt gene together with the construction of two counter selectable hprt-/- DT40 derived cell lines. One of these cell lines contains a stably integrated gene encoding a conditionally active cre recombinase and thus allows efficient manipulation of targeted loci by site-specific recombination. These cell lines will ...
متن کاملSHORT TECHNICAL REPORTS Counter-selectable marker for bacterial-based interaction trap systems
Many in vivo interaction trap systems have been developed for the identification of protein-protein, protein-DNA, and protein-RNA interactions. These methods typically involve the use of a “bait” (a specific protein, DNA, or RNA sequence) to identify an interacting partner from a library of different “prey.” These experiments can take the form of one-hybrid (1,2), twohybrid (3), or three-hybrid...
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ژورنال
عنوان ژورنال: FEMS Microbiology Letters
سال: 1998
ISSN: 0378-1097,1574-6968
DOI: 10.1111/j.1574-6968.1998.tb13097.x